PRIMER DESIGNING FOR PCR

PCR is a shorthand, simple but very useful procedure in molecular biology called the polymerase chain reaction. It is a technique used to amplify a segment of DNA of interest or produce lots and lots of copies. A primer…

Continue Reading PRIMER DESIGNING FOR PCR
Nitroblue Tetrazolium Assay(NBT) for estimation of superoxide anion in phagocytic cells
fig: A person performing NBT assay in laboratory

Nitroblue Tetrazolium Assay(NBT) for estimation of superoxide anion in phagocytic cells

The NBT assay is used to estimate the ability of cells to produce reactive oxygen species(ROS). This assay is mainly used in immunology for the semiquantitative measurement of ROS produced…

Continue Reading Nitroblue Tetrazolium Assay(NBT) for estimation of superoxide anion in phagocytic cells